Hungarian Centre of Excellence
for Molecular Medicine
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HCEMM-USZ Functional Cell Biology
and Immunology
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HCEMM-BRC Single Cell Omics
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HCEMM-SU In Vivo Imaging
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Description

of the tools

Mass spectrometry

Orbitrap Fusion Lumos Tribrid mass spectrometer with ETD (Thermo)

High resolution, high mass accuracy tribrid linear ion trap-quadrupole-Orbitrap mass spectrometer with CID, HCD and ETD dissociation techniques and MSn capabilities.

Orbitrap Elite mass spectrometer (Thermo)

High resolution, high mass accuracy hybrid linear ion trap-Orbitrap mass spectrometer with CID and HCD dissociation techniques and MSn capabilities.

Liquid chromatography

Evosep One

A high-throughput and robust platform using disposable trap columns enabling cross-contamination free analysis of up to 100 samples per day. We use this instrument interfaced to the Orbitrap Fusion Lumos for LC-MS/MS

Thermo Ultimate3000 RSLC

Nano LC System interfaced directly to the Orbitrap Fusion Lumos in projects requiring tunable LC gradients

Waters MClass nano-UPLC

Nano LC System interfaced directly to the Orbitrap Elite for LC-MS/MS

Waters nanoAcquity UPLC

Nano LC System interfaced directly to the Orbitrap Elite for LC-MS/MS

Offline sample fractionation

semi-micro (Jasco) and micro (Eldex)HPLC systems

Other sample introduction

Advion Triversa Nanomate

Chip-based automated electrospray ionization module for direct infusion

Software

Protein Prospector, Proteome Discoverer, Byonic

Chromium Controller (10xGenomics)

Microfluidic device enabling single cell partitioning and barcoding of cells for single cell transcriptomics projects 

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